Lys-63-specific deubiquitinase BRCC36 Polyclonal Antibody

Catalog Number:

42084

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P46736

Form of Antibody:

Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Storage/Stability:

Immunogen:

Recombinant human Lys-63-specific deubiquitinase BRCC36 protein

Purification:

Caprylic Acid Ammonium Sulfate Precipitation purified

Specificity/Sensitivity:

The antibody detects endogenous level of total Lys-63-specific deubiquitinase BRCC36 polyclonal antibody.

Applications:

WB

Background:

Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex.

References:

[1]Isolation and sequence of two genes associated with a CpG island 5' of the factor VIII gene.Kenwrick S., Levinson B., Taylor S., Shapiro A., Gitschier J.Hum. Mol. Genet. 1:179-186(1992)[2]The chromosomal translocation t(X;14)(q28;q11) in T-cell pro-

appl_detail:

Western blotting: 1:500 - 1:1000

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