Cleaved PARP Mouse Monoclonal Antibody

Catalog Number:

38076

Amount:

100μg

Price:

$369

Swiss-Prot No:

Swiss-Prot#:P42574

Form of Antibody:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Purification:

Affinity purification using immunogen.

Specificity/Sensitivity:

Antibody detects endogenous pro and active PARP protein.

Applications:

WB,IHC,IF

Background:

Poly [ADP-ribose] polymerase 1 (PARP-1) also known as NAD+ ADP-ribosyltransferase 1 or poly[ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks. Knocking down intracellular PARP1 levels with siRNA or inhibiting PARP1 activity with small molecules reduces repair of ssDNA breaks. In the absence of PARP1, when these breaks are encountered during DNA replication, the replication fork stalls, and double-strand DNA (dsDNA) breaks accumulate.

References:

appl_detail:

WB dilution: 1:2000~1:5000
IHC dilution:1:50-300
IF dilution:1:50-200

Datasheet PDF