Ku70 Monoclonal Antibody

Catalog Number:

27220

Amount:

100μg

Price:

$369

Swiss-Prot No:

Uniprot: P12956

Gene ID: 2547

Form of Antibody:

Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Purified recombinant human Ku70 protein fragments expressed in E.coli.

Purification:

Affinity purified

Specificity/Sensitivity:

This antibody detects endogenous levels of Ku70 and does not cross-react with related proteins.

Applications:

WB ICC IP

Background:

Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.

References:

appl_detail:

Western blotting: 1:1000

Immunocytochemistry: 1:200

Datasheet PDF