RPA70 Monoclonal Antibody

Catalog Number:

27212

Amount:

100μg

Price:

$369

Swiss-Prot No:

Uniprot: P27694

Gene ID: 6117

Form of Antibody:

Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Purified recombinant human RPA70 protein fragments expressed in E.coli.

Purification:

Affinity purified

Specificity/Sensitivity:

This antibody detects endogenous levels of RPA70 and does not cross-react with related proteins.

Applications:

WB ICC IP

Background:

 Plays an essential role in several cellular processes in DNA metabolism including replication,recombination and DNA repair.Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.Functions as component of the alternative replication protein A complex (aRPA).aRPA binds single-stranded DNA and probably plays a role in DNA repair;it does not support chromosomal DNA replication and cell cycle progression through S-phase.In vitro,aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC),the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

References:

appl_detail:

Western blotting: 1:1000

Immunocytochemistry: 1:100

Datasheet PDF