PCNA Monoclonal Antibody

Catalog Number:

27210

Amount:

100μg

Price:

$369

Swiss-Prot No:

Uniprot: P12004

Gene ID: 5111

Form of Antibody:

ascites

Storage/Stability:

Immunogen:

Purified recombinant human PCNA protein fragments expressed in E.coli.

Purification:

Specificity/Sensitivity:

This antibody detects endogenous levels of PCNA and does not cross-react with related proteins.

Applications:

WB IP ICC IHC

Background:

 Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase,but not apurinic-apyrimidinic (AP) endonuclease,APEX2 activities.Has to be loaded onto DNA in order to be able to stimulate APEX2.Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways.Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair:Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases,while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

References:

appl_detail:

Western blotting: 1:1000

Immunocytochemistry: 1:100

Datasheet PDF