EGFP/EYFP Monoclonal Antibody

Catalog Number:

27209

Amount:

100μg

Price:

$369

Swiss-Prot No:

Uniprot: C8CHS1

Form of Antibody:

Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Purified recombinant EYFP.tag full length expressed in E.coli.

Purification:

Affinity purified

Specificity/Sensitivity:

Transfected

Applications:

WB

Background:

 The green fluorescent protein (GFP) is a protein isolated from the jellyfish Aequorea victoria composed of 238 amino acid residues that exhibits bright green fluorescence. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations. Due to the potential for widespread usage and the evolving needs of researchers, many different mutants of GFP have been engineered. For example, EGFP contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr which result in  brighter green fluorescence. EYFP contains the four amino acid substitutions of Ser-65 to Gly; Val-68 to Leu; Ser-72 to Ala; and Thr-203 to Tyr. EYFP emits yellow fluorescence excited by green light.

References:

appl_detail:

 Western blotting: 1:1000

Datasheet PDF