RAD9A Monoclonal Antibody

Catalog Number:

27193

Amount:

100μg

Price:

$369

Swiss-Prot No:

Uniprot: Q99638

Gene ID: 5883

Form of Antibody:

Purified mouse monoclonal in PBS(pH 7.4)containing with 0.2% sodium azide,50% glycerol.

Storage/Stability:

Immunogen:

Purified recombinant human Rad9A protein fragments expressed in E.coli

Purification:

Affinity purified

Specificity/Sensitivity:

This antibody detects endogenous levels of RAD9A,and does not cross-react with related proteins.

Applications:

WB IP

Background:

 Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair.The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C(RFC)clamp loader complex.Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair(LP-BER).The 9-1-1 complex stimulates DNA polymerase beta(POLB)activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds;endonuclease FEN1 cleavage activity on substrates with double,nick,or gap flaps of distinct sequences and lengths;and DNA ligase I(LIG1)on long-patch base excision repair substrates.The 9-1-1 complex is necessary for the recruitment of C12orf32/RHINO to sites of double-stranded breaks(DSB)occurring during the S phase.RAD9A possesses 3'->5' double stranded DNA exonuclease activity.Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

References:

appl_detail:

 Western blotting: 1:500

Datasheet PDF