SRGAP1 Antibody

Catalog Number:

40222

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot:Q7Z6B7
Gene Accssion:NP_065813

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Synthetic peptide corresponding to residues near the C terminal of human SLIT-ROBO Rho GTPase activating protein 1

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects endogenous levels of total SRGAP1 protein.

Applications:

WB IHC

Background:

SRGAP1 contains an FCH (Fes/CIP4 homology) domain, a Rho-GAP domain and an SH3 domain. In the presence of Slit, SRGAP1 (via its SH3 domain) binds to the CC3 motif in robo (a protein responsible for mediating the repulsive effect of Slit) with higher affinity and inhibits Cdc42 activity in a robo/SRGAP-dependent manner. More specifically, SRGAP1 increases the intrinsic GTPase activity of Cdc42, thereby converting it to its inactive, GDP-bound form. Inactivation of Cdc42 ultimately leads to a decrease in actin polymerization.

References:

appl_detail:

Western blotting: 1:500-1:2000
Immunohistochemistry: 1:100-1:200

Datasheet PDF