Her2 Monoclonal Antibody

Catalog Number:

27150

Amount:

100μg

Price:

$369

Swiss-Prot No:

Swiss-Prot#: P04626

Form of Antibody:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Purified recombinant fragment of human Her2 expressed in E. Coli

Purification:

Affinity-chromatography

Specificity/Sensitivity:

Her2 antibody detects endogenous levels of total Her2

Applications:

ELISA WB IHC

Background:

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

References:

appl_detail:

 ELISA: 1:10000

   WB 1:500 - 1:2000

 IHC 1:200 - 1:1000

Datasheet PDF