E2F1 antibody

Catalog Number:

38351

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q01094
NCBI Gene ID: 1869

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

A synthetic peptide of human E2F1.

Purification:

Antibodies were purified by affinity purification using immunogen.

Specificity/Sensitivity:

The antibody detects endogenous level of total E2F1 antibody.

Applications:

WB,IF

Background:

The E2F transcription factors are essential for regulation of the cell cycle (1,2). Physiological E2F is a heterodimer composed of an E2F subunit together with a DP subunit (3, 4). Six members of the E2F family have been identified, and each E2F subunit has a DNA binding and a dimerization domain. E2F-1 to -5 activate transcription. E2F-1 to -3 bind pRb, and E2F-4 and -5 bind p107 or p130, and these interactions are under cell cycle control (5-8). E2F-1 has oncogenic properties in vivo and in vitro. E2F-1 can induce apoptosis through p53-dependent and -independent mechanisms. E2F-1 is stress-responsive, and is regulated by a PI3-kinase-like kinase family such as the ATM/ATR kinases (9-11).

References:

appl_detail:

WB 1:500 - 1:1000
IF 1:20 - 1:100

Datasheet PDF