HSPA5 antibody

Catalog Number:

38110

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P11021
NCBI Gene ID: 3309

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Recombinant protein of human HSPA5.

Purification:

Antibodies were purified by affinity purification using immunogen.

Specificity/Sensitivity:

The antibody detects endogenous level of total HSPA5 antibody.

Applications:

WB,IHC,IF

Background:

Secretory and transmembrane proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside the ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including HSPA5. HSPA5 was identified as an immunoglobulin heavy chain binding protein in pre-B cells (1,2). It was also found to be induced at the protein level by glucose starvation (3). When protein folding is disturbed inside ER, HSPA5 synthesis is increased. Subsequently, HSPA5 binds to misfolded proteins to prevent them from forming aggregates and assists in proper refolding (4).

References:

appl_detail:

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Datasheet PDF