PKR(Phospho-Thr446) Antibody

Catalog Number:

11280

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P19525
NCBI Protein: NP_001129123.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 446 (K-R-T(p)-R-S) derived from Human PKR.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of PKR only when phosphorylated at threonine 446.

Applications:

WB

Background:

Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.

Abujiang Pataer, et,al. (2002) Cancer Res; 62: 2239.
K. D. Ryman, et,al. (2005) J. Virol; 79: 1487 - 1499.
Susana Guerra, et,al. (2006) J. Biol. Chem; 281: 18734 - 18745.
 

References:

appl_detail:

Predicted MW: 68kd
Western blotting: 1:500~1:1000

Datasheet PDF