eIF2a(Phospho-Ser51) Antibody

Catalog Number:

11279

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P05198
NCBI Protein: NP_004085.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 51 (E-L-S(p)-R-R) derived from Human eIF2a.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of eIF2a only when phosphorylated at serine 51.

Applications:

WB IHC IF

Background:

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Xavier Saelens, et al. (2001) J. Biol. Chem; 276: 41620 - 41628.
Hiroyuki Kubota, et al. (2003) J. Biol. Chem ; 278: 20457 - 20460.
Shijian Chu, et al. (2006) Am J Physiol Lung Cell Mol Physiol ; 291: L983 - L992.
Eileen Connolly, et al. (2006) Mol. Cell. Biol ; 26: 3955 - 3965.

References:

appl_detail:

Predicted MW: 38kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Datasheet PDF