EGFR(phospho-Tyr1110) Antibody

Catalog Number:

11264

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P00533
NCBI Protein: NP_005219.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 1110 (P-V-Y(p)-H-N) derived from Human EGFR.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of EGFR only when phosphorylated at tyrosine 1110.

Applications:

WB

Background:

Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.

Kaisa Erjala, et,al. (2006) Clin. Cancer Res Jul 2006; 12: 4103 - 4111.
Judit Anido, et,al. (2003) Clin. Cancer Res ; 9: 1274.
Julian Andreev, et,al. (2001) J. Biol. Chem ; 276: 20130 - 20135.
Suil Kim, et,al. (2002) Am J Physiol Lung Cell Mol Physiol, ; 283: 67.

References:

appl_detail:

Predicted MW: 175kd
Western blotting: 1:500~1:1000

Datasheet PDF