Connexin 43 (phospho-Ser368) Antibody

Catalog Number:

11258

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P17302
NCBI Protein: NP_000156.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 368 (R-A-S(p)-S-R) derived from Human Connexin 43.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of Connexin43 only when phosphorylated at serine 368.

Applications:

WB IHC

Background:

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.

Joell L. Solan1, et al. (2003) Cell Science 116: 2203-2211
Satoshi Matsushita, et al. (2006) Histochemistry and Cytochemistry 54 (3): 343-353,
Xiaoyong Bao, et al. (2004) Cell Physiol 286: C647-C654
W. E. I. Li, et al.(1998) European Journal of Neuroscience 10: 2444
 

References:

appl_detail:

Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
 

Datasheet PDF