ADD2 Antibody

Catalog Number:

36050

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P35612
NCBI Gene ID: 119
Gene Accssion: BC041666

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Fusion protein corresponding to residues near the C terminal of human adducin 2 (beta)

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects endogenous levels of total ADD2 protein.

Applications:

IHC

Background:

Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described.

References:

appl_detail:

Immunohistochemistry: 1:15-1:50

Datasheet PDF