HDAC4(Phospho-Ser632) Antibody

Catalog Number:

11192

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P56524
NCBI Protein: NP_006028.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of serine 632 (A-Q-S(p)-S-P) derived from Human HDAC4.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of HDAC4 only when phosphorylated at serine 632.

Applications:

WB

Background:

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.

Wang AH, et al. (2000) Mol Cell Biol. 20(18): 6904-6912.
Grozinger CM, et al. (2000) Proc Natl Acad Sci U S A. 97(14): 7835-7840.
 

References:

appl_detail:

Predicted MW: 140kd
Western blotting: 1:500~1:1000
 

Datasheet PDF