DNA PKcs(Phospho-Thr2609) Antibody

Catalog Number:

11167

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P78527
NCBI Protein: NP_001075109.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 2609 (V-E-T(p)-Q-A) derived from Human DNA-PK.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of DNA PKcs only when phosphorylated at threonine 2609.

Applications:

WB

Background:

The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties

Chan DW, et al. (2002) Genes Dev. Sep 15; 16(18): 2333-2338
Ding Q, et al. (2003) Mol Cell Biol. Aug; 23(16): 5836-5848
Douglas P, et al. (2002) Biochem J. Nov 15; 368(Pt 1): 243-251

References:

appl_detail:

Predicted MW: 450kd
Western blotting: 1:500~1:1000

Datasheet PDF