Vav(Phospho-Tyr174) Antibody

Catalog Number:

11142

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P15498
NCBI Protein: NP_005419.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 174 (E-I-Y(p)-E-D) derived from Human Vav.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of Vav only when phosphorylated at tyrosine 174.

Applications:

WB

Background:

Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation

Amarasinghe GK, et al.

References:

appl_detail:

Predicted MW: 95kd
Western blotting: 1:500~1:1000

Datasheet PDF