CDC27 Antibody

Catalog Number:

35675

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: P30260
NCBI Gene ID: 996
Gene Accssion: BC011656

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Fusion protein corresponding to residues near the C terminal of human cell division cycle 27

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects endogenous levels of total CDC27 protein.

Applications:

IHC

Background:

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.?

References:

appl_detail:

Immunohistochemistry: 1:50-1:200

Datasheet PDF