NFKBIE Antibody

Catalog Number:

35589

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: O00221
NCBI Gene ID: 4794
Gene Accssion: BC011676

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Fusion protein corresponding to a region derived from internal residues of human Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects endogenous levels of total NFKBIE protein.

Applications:

WB IHC

Background:

The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. NFKBIE protein expression is up-regulated following NF-κB activation and during myelopoiesis. NFKBIE is able to inhibit NF-κB-directed transactivation via cytoplasmic retention of REL proteins. NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NF-κB transcription factor complex. The NF-κB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm.

References:

appl_detail:

Western blotting: 1:200-1:1000
Immunohistochemistry: 1:50-1:200

Datasheet PDF