ICAM-1(Phospho-Tyr512) Antibody

Catalog Number:

11083

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P05362
NCBI Protein: NP_000192.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 512 (K-K-Y(p)-R-L) derived from Human ICAM-1.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of ICAM-1 only when phosphorylated at tyrosine 512.

Applications:

WB

Background:

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin a-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

Greenwood J, et al. (2003) J Immunol; 171(4):2099-2108.
Zhou Z, et al. (2005) Eur J Pharmacol; 513(1-2):1-8.
Chen YH, et al. (2001) J Cell Biochem; 82(3):512-521
 

References:

appl_detail:

Predicted MW: 89 92kd
Western blotting: 1:500~1:1000
 

Datasheet PDF