EGFR(Phospho-Tyr1092) Antibody

Catalog Number:

11081

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P00533
NCBI Protein: NP_005219.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine1092 (P-E-Y(p)-I-N) derived from Human EGFR.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of EGFR only when phosphorylated at tyrosine 1092.

Applications:

WB IHC

Background:

Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.

Buerger C, et al. (2003) J Biol Chem; 278(39): 37610-21.
Wang XQ, (2003) J Biol Chem; 278(49): 48770-8.
Saito T, et al. (2004) Endocrinology; 145(9): 4232-43.
Pao W, et al. (2004) Proc Natl Acad Sci U S A; 101(36): 13306-11.

References:

appl_detail:

Predicted MW: 175kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF