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FLAG tag Antibody

Catalog Number:

35535
other_names: DDDDK epitope tag; DYKDDDDK epitope tag

Amount:

100μg
calculated_mw:
host_species: Rabbit

Price:

$319

Swiss-Prot No:

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Synthetic peptide: DYKDDDDK conjugated to KLH

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects transfected proteins contanining FLAG tag.

Applications:

WB Elisa

Background:

The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins.

References:

appl_detail:

Western blotting: 1:5000-1:20000

ELISA dilution: 1:5000-1:10000

Datasheet PDF

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