Integrin b3(Phospho-Tyr773) Antibody

Catalog Number:

11060

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P05106
NCBI Protein: NP_000203.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 773 (P-L-Y(p)-K-E) derived from Human Integrin b3.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of Integrin b3 only when phosphorylated at tyrosine 773.

Applications:

WB IHC

Background:

Integrin a-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin a-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins a-IIb/beta-3 and a-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin a-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin a-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Chandhoke SK, et al. (2004) J Cell Sci.117 (Pt 8): 1431-1441.
Datta A, et al. (2002) J Biol Chem.277 (6): 3943-3949.
Law DA, et al. (1996) J Biol Chem.271 (18): 10811-10815.

References:

appl_detail:

Predicted MW: 110kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF