Akt(Phospho-Thr308) Antibody

Catalog Number:

11055

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P31749
NCBI Protein: NP_001014431.1

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of threonine 308 (M-K-T(p)-F-C) derived from Human Akt.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of Akt only when phosphorylated at threonine 308.

Applications:

WB IHC IF

Background:

General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.

Tremblay F, et al. (2005)Diabetes; 54(9): 2674-84.
Xu BE, et al. (2005)J Biol Chem; 280(40): 34218-23.
Samuels Y, et al. (2005)Cancer Cell; 7(6): 561-73.
Di Maira G, et al. (2005)Cell Death Differ; 12(6): 668-77.

References:

appl_detail:

Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Datasheet PDF