STAT5a(Phospho-Tyr694) Antibody

Catalog Number:

11048

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P42229
NCBI Protein: NP_003143.2

Form of Antibody:

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 694 (D-G-Y(p)-V-K) derived from Human STAT5A.

Purification:

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Specificity/Sensitivity:

The antibody detects endogenous level of STAT5A only when phosphorylated at tyrosine 694.

Applications:

WB IHC

Background:

Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.

Gouilleux F, et al. (1994) EMBO J. 13: 4361-4369.
Dentelli P, et al. (1999) J Immunol. 163: 2151-2159.
Meinke A, et al. (1996) Mol Cell Biol. 16: 6937-6944.

References:

appl_detail:

Predicted MW: 90kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Datasheet PDF