ERI1 Antibody

Catalog Number:

35304

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q8IV48
NCBI Gene ID: 90459

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human ERI1.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total ERI1 protein.

Applications:

WB

Background:

RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.

Dominski Z., Mol. Cell 12:295-305(2003).
Ota T., Nat. Genet. 36:40-45(2004).
Bechtel S., BMC Genomics 8:399-399(2007)

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF