MRCKa Antibody

Catalog Number:

35291

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q5VT25
NCBI Gene ID: 8476

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from Internal of human MRCKa.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total MRCKa protein.

Applications:

WB IHC IF

Background:

Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates: PPP1R12A, LIMK1 and LIMK2. May play a role in TFRC-mediated iron uptake.

Wilkinson S., Nat. Cell Biol. 7:255-261(2005).
Totoki Y., Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
Bechtel S., BMC Genomics 8:399-399(2007).

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF