NDFIP1 Antibody

Catalog Number:

35199

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q9BT67
NCBI Gene ID: 80762

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from C-terminal of human NDFIP1.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total NDFIP1 protein.

Applications:

WB

Background:

Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation By similarity. In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity. Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate.

Petroziello J.M., Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
Matsuda A., Oncogene 22:3307-3318(2003).
Otsuki T., DNA Res. 12:117-126(2005).

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF