UBE2T Antibody

Catalog Number:

35133

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q9NPD8
NCBI Gene ID: 29089

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human UBE2T.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total UBE2T protein.

Applications:

WB

Background:

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair: acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination.

Okaze H., Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
Hu R.-M., Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
Zhang Q.-H., Genome Res. 10:1546-1560(2000).

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF