Raf1 Antibody

Catalog Number:

35408

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#:P04049;
NCBI Gene#:5894

Form of Antibody:

Rabbit IgG in 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage/Stability:

Immunogen:

Recombinant fragment corresponding to a region within amino acids 1 and 265 of Raf1.

Purification:

Antibodies were purified by antigen-affinity chromatography.

Specificity/Sensitivity:

The antibody detects endogenous levels of total Raf1 protein.

Applications:

IF

Background:

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq]

References:

appl_detail:

IF 1:50 - 1:100

Datasheet PDF