PP4R2 Antibody

Catalog Number:

35035

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q9NY27
NCBI Gene ID: 151987

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human PP4R2.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total PP4R2 protein.

Applications:

WB

Background:

Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Mediates RPA2 dephosphorylation by recruiting PPP4C to RPA2 in a DNA damage-dependent manner. RPA2 dephosphorylation is required for the efficient RPA2-mediated recruitment of RAD51 to chromatin following double strand breaks, an essential step for DNA repair.

Hastie C.J., Biochem. J. 347:845-855(2000).
Zhang W., Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases.
Mural R.J., Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF