RFWD3Antibody

Catalog Number:

35004

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q6PCD5
NCBI Gene ID: 55159

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human RFWD3.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total RFWD3 protein.

Applications:

WB

Background:

E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. Plays a role in RPA-mediated DNA damage signaling and repair.

The MGC Project Team, Genome Res. 14:2121-2127(2004).
Ota T., Nat. Genet. 36:40-45(2004).
Matsuoka S., Science 316:1160-1166(2007).

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF