MED27 Antibody

Catalog Number:

35183

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q6P2C8
NCBI Gene ID: 9442

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from Internal of human MED27.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total MED27 protein.

Applications:

WB

Background:

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Humphray S.J., Nature 429:369-374(2004).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Ryu S., Nature 397:446-450(1999).

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF