RAD18 Antibody

Catalog Number:

34662

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q9NS91
NCBI Gene ID: 56852

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human RAD18.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total RAD18 protein.

Applications:

WB

Background:

E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.

Tateishi S., Proc. Natl. Acad. Sci. U.S.A. 97:7927-7932(2000).
Xin H., Nucleic Acids Res. 28:2847-2854(2000).
Muzny D.M., Nature 440:1194-1198(2006).

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF