POLR1B Antibody

Catalog Number:

34651

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q9H9Y6
NCBI Gene ID: 84172

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from C-terminal of human POLR1B.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total POLR1Bprotein.

Applications:

WB

Background:

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft By similarity.

Ota T., Nat. Genet. 36:40-45(2004).
Hillier L.W., Nature 434:724-731(2005).
Miller G., EMBO J. 20:1373-1382(2001).

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF