ATP5A1 Antibody

Catalog Number:

34453

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P25705
NCBI Gene ID: 498

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human ATP5A1.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total ATP5A1 protein.

Applications:

WB

Background:

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites By similarity.

Kataoka H., Biochim. Biophys. Acta 1089:393-395(1991).
Godbout R., Gene 123:195-201(1993).
Akiyama S., Biochim. Biophys. Acta 1219:129-140(1994)

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF