AZI1 Antibody

Catalog Number:

34342

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q9UPN4
NCBI Gene ID: 22994

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human AZI1.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total AZI1 protein.

Applications:

WB IHC

Background:

Component of centriolar satellites, which build a complex and dynamic network required to regulate cilia/flagellum formation. During normal and stress-induced ciliogenesis, its non-ubiquitinated form is rapidly displaced from centriolar satellites and recruited to centrosome/basal bodies in a p38 MAPK-dependent manner. In contrast, in proliferating cells, MIB1-mediated ubiquitination induces its sequestration within centriolar satellites, precluding untimely cilia formation initiation. Acts also as a negative regulator for the trafficking from the centriolar satellite BBSome protein complex to the cilia. Plays a role in sperm flagellar formation; may be involved in the regulation of intraflagellar transport (IFT) and/or intramanchette (IMT) trafficking, which are important for axoneme extension and/or cargo delivery to the nascent sperm tail. Required for optimal cell proliferation and cell cycle progression; may play a role in the regulation of genome stability and centriole duplication in non-ciliogenic cells.

Kikuno R., DNA Res. 6:197-205(1999).
Andersen J.S., Nature 426:570-574(2003).
Olsen J.V., Cell 127:635-648(2006).

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF