RAD21 Antibody

Catalog Number:

34296

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: O60216
NCBI Gene ID: 5885

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human RAD21.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total RAD21 protein.

Applications:

WB

Background:

Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.

McKay M.J., Genomics 36:305-315(1996).
Sadano H., Biochem. Biophys. Res. Commun. 267:418-422(2000).
Nomura N., DNA Res. 1:223-229(1994).

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF