MRE11A Antibody

Catalog Number:

34288

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P49959
NCBI Gene ID: 4361

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human MRE11A.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total MRE11A protein.

Applications:

WB

Background:

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.

Petrini J.H.J.,, Genomics 29:80-86(1995).
Paull T.T., Mol. Cell 1:969-979(1998).
Pitts S.A., Hum. Mol. Genet. 10:1155-1162(2001).

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF