DNL4 Antibody

Catalog Number:

34266

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P49917
NCBI Gene ID: 3981

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human DNL4.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total DNL4 protein.

Applications:

WB

Background:

Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

Wei Y.-F., Mol. Cell. Biol. 15:3206-3216(1995).
Dunham A., Nature 428:522-528(2004).
Robins P., J. Biol. Chem. 271:24257-24261(1996).

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF