Cytochrome P450 3A4 Antibody

Catalog Number:

34243

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P08684
NCBI Gene ID: 1576

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human Cytochrome P450 3A4.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total Cytochrome P450 3A4 protein.

Applications:

WB IHC

Background:

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2-exo-monooxygenase. The enzyme also hydroxylates etoposide.

Vuoristo M.M., J. Biol. Chem. 270:22873-22881(1995).
Mungall A.J., Nature 425:805-811(2003).
Lui V.C., Genomics 32:401-412(1996).

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Datasheet PDF