CDC20 Antibody

Catalog Number:

34189

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q12834
NCBI Gene ID: 991

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human CDC20.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total CDC20 protein.

Applications:

WB

Background:

Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.

Weinstein J., Mol. Cell. Biol. 14:3350-3363(1994).
Kramer E.R., Curr. Biol. 8:1207-1210(1998).
Gregory S.G., Nature 441:315-321(2006).

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF