Histone H2B Antibody

Catalog Number:

34015

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q93079
NCBI Gene ID: 8345

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human Histone H2B.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total Histone H2B protein.

Applications:

WB IHC IF

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Albig W., Genomics 40:314-322(1997).
Marzluff W.F., Genomics 80:487-498(2002).
Mungall A.J., Nature 425:805-811(2003).

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Datasheet PDF