SGOL1 Antibody

Catalog Number:

33926

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q5FBB7
NCBI Gene ID: 151648

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human SGOL1.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total SGOL1 protein.

Applications:

WB

Background:

Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity.

McGuinness B.E., PLoS Biol. 3:433-449(2005).
Kitajima T.S., Curr. Biol. 15:353-359(2005).
Tang Z., Proc. Natl. Acad. Sci. U.S.A. 101:18012-18017(2004).

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF