CAF1A Antibody

Catalog Number:

33866

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q13111
NCBI Gene ID: 10036

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human CAF1A.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total CAF1A protein.

Applications:

WB IHC IF

Background:

Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci By similarity. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Dong H., Gene 264:187-196(2001) [PubMed: 11250073].
Grimwood J., Nature 428:529-535(2004) [PubMed: 15057824].
Kaufman P.D., Cell 81:1105-1114(1995) [PubMed: 7600578].

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF