IκB-α Antibody

Catalog Number:

33726

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P25963
NCBI Gene ID: 4792

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from N-terminal of human IκB-α.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total IκB-α protein.

Applications:

WB

Background:

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Haskill S., Cell 65:1281-1289(1991).
Jungnickel B., J. Exp. Med. 191:395-402(2000).
Liu B., Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases.

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF