Cytochrome P450 1A2 Antibody

Catalog Number:

34228

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P05177
NCBI Gene ID: 1544

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human Cytochrome P450 1A2.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total Cytochrome P450 1A2 protein.

Applications:

IHC

Background:

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin.

Jaiswal A.K., Nucleic Acids Res. 14:6773-6774(1986).
Corchero J., Pharmacogenetics 11:1-6(2001).
Soyama A.,Drug Metab. Pharmacokinet. 20:24-33(2005).

References:

appl_detail:

Immunohistochemistry: 1:50~1:100

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