TBP Antibody

Catalog Number:

33709

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: P20226
NCBI Gene ID: 6908

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human TBP.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total TBP protein.

Applications:

WB

Background:

General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.

Peterson M.G., Science 248:1625-1630(1990).
Mungall A.J., Nature 425:805-811(2003).
Gangloff Y.G., Mol. Cell. Biol. 21:5109-5121(2001).

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF